Nop132 Polyclonal Antibody
- 货号：YT3165
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Nucleolar protein 8
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NOL8. AA range:1118-1167
- 特异性：
- Nop132 Polyclonal Antibody detects endogenous levels of Nop132 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NOL8;C9orf34;NOP132;Nucleolar protein 8;Nucleolar protein Nop132
- 背景：
- NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM, Mar 2008],
- 功能：
- function:Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.,induction:Up-regulated in diffuse-type gastric cancers.,PTM:Phosphorylated.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7.,tissue specificity:Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.,
- 细胞定位：
- Nucleus, nucleolus . Localizes in the nucleolar-organizing region during ribosome biogenesis.
- 组织表达：
- Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
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- Western Blot analysis of 3T3 cells using Nop132 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of NIH-3T3 cells using Nop132 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of NOL8 Antibody. The lane on the right is blocked with the NOL8 peptide.
- Immunohistochemistryt analysis of paraffin-embedded human brain, using NOL8 Antibody. The lane on the right is blocked with the NOL8 peptide.
- Western blot analysis of the lysates from HepG2 cells using NOL8 antibody.
