NOL10 Polyclonal Antibody
- 货号:YT3163
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Nucleolar protein 10
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NOL10. AA range:639-688
- 特异性:
- NOL10 Polyclonal Antibody detects endogenous levels of NOL10 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NOL10;Nucleolar protein 10
- 背景:
- similarity:Belongs to the WD repeat NOL10/ENP2 family.,similarity:Contains 5 WD repeats.,
- 功能:
- similarity:Belongs to the WD repeat NOL10/ENP2 family.,similarity:Contains 5 WD repeats.,
- 细胞定位:
- Nucleus, nucleolus .
![](/uploadfiles/PIC/yt3163-wb.jpg)
- Western Blot analysis of various cells using NOL10 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt3163-ihc-824.jpg)
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
![](/uploadfiles/PIC/yt3163_pic724618522.jpg)
- Western blot analysis of lysates from HUVEC cells, using NOL10 Antibody. The lane on the right is blocked with the synthesized peptide.