NFATc4 Polyclonal Antibody

    • 货号:YT3085
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • NFAT3
      • 简介:
      • >>cGMP-PKG signaling pathway;>>Cellular senescence;>>Wnt signaling pathway;>>Axon guidance;>>C-type lectin receptor signaling pathway;>>Oxytocin signaling pathway;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Human immunodeficiency virus 1 infection
      • 基因名称:
      • NFATC4
      • 蛋白名称:
      • Nuclear factor of activated T-cells cytoplasmic 4
      • Human Gene Id:
      • 4776
      • Human Swiss Prot No:
      • Q14934
      • Mouse Swiss Prot No:
      • Q8K120
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human NFAT3. AA range:642-691
      • 特异性:
      • NFATc4 Polyclonal Antibody detects endogenous levels of NFATc4 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NFATC4;NFAT3;Nuclear factor of activated T-cells; cytoplasmic 4;NF-ATc4;NFATc4;T-cell transcription factor NFAT3;NF-AT3
      • 实测条带:
      • 135kD
      • 背景:
      • This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
      • 功能:
      • domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.,PTM:Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by FRAP1, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.,PTM:Ubiquitinated, leading
      • 细胞定位:
      • Cytoplasm, cytosol . Nucleus . When hyperphosphorylated, localizes in the cytosol. When intracellular Ca(2+) levels increase, dephosphorylation by calcineurin/PPP3CA leads to translocation into the nucleus (PubMed:11997522, PubMed:18347059). MAPK7/ERK5 and MTOR regulate NFATC4 nuclear export through phosphorylation at Ser-168 and Ser-170 (PubMed:18347059). .
      • 组织表达:
      • Widely expressed, with high levels in placenta, lung, kidney, testis and ovary (PubMed:18675896). Weakly expressed in spleen and thymus (PubMed:18675896). In the hippocampus, expressed in the granular layer of the dentate gyrus, in the pyramidal neurons of CA3 region, and in the hippocampal fissure (PubMed:18675896). Expressed in the heart (at protein level) (PubMed:12370307).

      Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels. BMC CANCER Bmc Cancer. 2021 Dec;21(1):1-14 IHC Human H1299 cell
      货号:YT3085

      • 产品图片
      • Western Blot analysis of various cells using NFATc4 Polyclonal Antibody diluted at 1:500
      • Immunofluorescence analysis of HeLa cells, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, using NFAT3 Antibody. The lane on the right is blocked with the synthesized peptide.