Nesprin-3 Polyclonal Antibody
- 货号：YT3039
- 应用：WB;IF;ELISA
- 种属：Human;Mouse
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human C14orf49. AA range:169-218
- 特异性：
- Nesprin-3 Polyclonal Antibody detects endogenous levels of Nesprin-3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SYNE3;C14orf49;Nesprin-3;Nuclear envelope spectrin repeat protein 3
- 背景：
- function:Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which theters the nucleus to the cytoskeleton by binding PLEC1/Plectin which can associate with the intermediate filament system.,similarity:Belongs to the nesprin family.,similarity:Contains 1 KASH domain.,similarity:Contains 2 spectrin repeats.,subcellular location:Located at the rough reticulum endoplasmic (RER) in some cells.,subunit:Interacts with actin-binding domain of PLEC1/plectin.,
- 功能：
- function:Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which theters the nucleus to the cytoskeleton by binding PLEC1/Plectin which can associate with the intermediate filament system.,similarity:Belongs to the nesprin family.,similarity:Contains 1 KASH domain.,similarity:Contains 2 spectrin repeats.,subcellular location:Located at the rough reticulum endoplasmic (RER) in some cells.,subunit:Interacts with actin-binding domain of PLEC1/plectin.,
- 细胞定位：
- Nucleus outer membrane ; Single-pass type IV membrane protein . Nucleus envelope . Rough endoplasmic reticulum .
- 组织表达：
- Expressed in aortic endothelial cells (at protein level).
- Western Blot analysis of Jurkat cells using Nesprin-3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat cells, using C14orf49 Antibody. The lane on the right is blocked with the synthesized peptide.
