Neddylin Polyclonal Antibody
- 货号：YT3026
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat;Monkey
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NEDD8. AA range:10-59
- 特异性：
- Neddylin Polyclonal Antibody detects endogenous levels of Neddylin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NEDD8;NEDD8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8;NEDD-8;Ubiquitin-like protein Nedd8
- 背景：
- function:Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.,PTM:Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.,similarity:Belongs to the ubiquitin family.,subcellular location:Mainly nuclear.,subunit:Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.,tissue specificity:Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.,
- 功能：
- function:Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.,PTM:Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.,similarity:Belongs to the ubiquitin family.,subcellular location:Mainly nuclear.,subunit:Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.,tissue specificity:Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.,
- 细胞定位：
- Nucleus . Mainly nuclear. .
- 组织表达：
- Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
- Western Blot analysis of various cells using Neddylin Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.
