NCoA-7 Polyclonal Antibody
- 货号：YT2998
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Nuclear receptor coactivator 7
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NCOA7. AA range:422-471
- 特异性：
- NCoA-7 Polyclonal Antibody detects endogenous levels of NCoA-7 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NCOA7;ERAP140;ESNA1;Nbla00052;Nbla10993;Nuclear receptor coactivator 7;140 kDa estrogen receptor-associated protein;Estrogen nuclear receptor coactivator 1
- 背景：
- function:Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.,similarity:Belongs to the OXR1 family.,similarity:Contains 1 LysM repeat.,similarity:Contains 1 TLD domain.,subunit:Interacts with ESR1, ESR2A, ESR2B, THRB, PPARG and RARA in a ligand-inducible manner. Interacts with the heterodimer AHR-ARNT.,tissue specificity:Highly expressed in brain. Weakly expressed in mammary gland, ovary, uterus, prostate, stomach, bladder, spinal cord and pancreas. Expressed in cancer cell line.,
- 功能：
- function:Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.,similarity:Belongs to the OXR1 family.,similarity:Contains 1 LysM repeat.,similarity:Contains 1 TLD domain.,subunit:Interacts with ESR1, ESR2A, ESR2B, THRB, PPARG and RARA in a ligand-inducible manner. Interacts with the heterodimer AHR-ARNT.,tissue specificity:Highly expressed in brain. Weakly expressed in mammary gland, ovary, uterus, prostate, stomach, bladder, spinal cord and pancreas. Expressed in cancer cell line.,
- 组织表达：
- Highly expressed in brain. Weakly expressed in mammary gland, ovary, uterus, prostate, stomach, bladder, spinal cord and pancreas. Expressed in cancer cell line.
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from LOVO, NIH/3T3, and RAW264.7 cells, using NCOA7 Antibody. The lane on the right is blocked with the synthesized peptide.
