N4BP1 Polyclonal Antibody
- 货号：YT2962
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- NEDD4-binding protein 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
- 特异性：
- N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- N4BP1;KIAA0615;NEDD4-binding protein 1;N4BP1
- 背景：
- PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
- 功能：
- PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
- 细胞定位：
- Cytoplasm, cytosol . Nucleus . Nucleus, nucleolus . Nucleus, PML body . Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. .
- 组织表达：
- Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
- Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from MCF-7 and Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is blocked with the synthesized peptide.
