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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MYL6 Polyclonal Antibody
- 货号:YT2937
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>Vascular smooth muscle contraction;>>Tight junction;>>Oxytocin signaling pathway
- 蛋白名称:
- Myosin light polypeptide 6
- 免疫原:
- Synthesized peptide derived from the Internal region of human MYL6.
- 特异性:
- MYL6 Polyclonal Antibody detects endogenous levels of MYL6 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MYL6;Myosin light polypeptide 6;17 kDa myosin light chain;LC17;Myosin light chain 3;MLC-3;Myosin light chain alkali 3;Myosin light chain A3;Smooth muscle and nonmuscle myosin light chain alkali 6
- 背景:
- Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- function:Regulatory light chain of myosin. Does not bind calcium.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,
- 细胞定位:
- cytosol,brush border,membrane,myosin complex,unconventional myosin complex,extracellular matrix,vesicle,extracellular exosome,
- 组织表达:
- B-cell lymphoma,Brain,Duodenum,Eye,Lung,Placenta,
![](/uploadfiles/PIC/YT2937wb-2020.jpg)
- Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/28zhongfenhuaaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).