MSK1 Polyclonal Antibody

    • 货号:YT2902
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • MSK1
      • 简介:
      • >>MAPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TNF signaling pathway;>>Circadian entrainment;>>Neurotrophin signaling pathway;>>Shigellosis;>>Pathways in cancer;>>MicroRNAs in cancer;>>Bladder cancer
      • 基因名称:
      • RPS6KA5
      • 蛋白名称:
      • Ribosomal protein S6 kinase alpha-5
      • Human Gene Id:
      • 9252
      • Human Swiss Prot No:
      • O75582
      • Mouse Swiss Prot No:
      • Q8C050
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human MSK1. AA range:551-600
      • 特异性:
      • MSK1 Polyclonal Antibody detects endogenous levels of MSK1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RPS6KA5;MSK1;Ribosomal protein S6 kinase alpha-5;S6K-alpha-5;90 kDa ribosomal protein S6 kinase 5;Nuclear mitogen- and stress-activated protein kinase 1;RSK-like protein kinase;RSKL
      • 实测条带:
      • 90kD
      • 背景:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.,
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28'
      • 细胞定位:
      • Nucleus. Cytoplasm. Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
      • 组织表达:
      • Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

      Overexpressed mitogen-and stress-activated protein kinase 1 promotes the resistance of cytarabine in acute myeloid leukemia through brahma related gene 1-mediated upregulation of heme oxygenase-1. EUROPEAN JOURNAL OF PHARMACOLOGY Eur J Pharmacol. 2022 Feb;917:174722 WB,IF,ICC Human 1:1000,1:500 THP-1 cell, U937 cell
      货号:YT2902

      8-Oxoguanine DNA glycosylase 1 selectively modulates ROS-responsive NF-κB targets through recruitment of MSK1 and phosphorylation of RelA/p65 at Ser276. JOURNAL OF BIOLOGICAL CHEMISTRY Ruoxi Wang IP Human HEK293 cell
      货号:YT2902

      • 产品图片
      • Western Blot analysis of various cells using MSK1 Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HUVEC cells, using MSK1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, treated with UV 15', using MSK1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).