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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MPP9 Polyclonal Antibody
- 货号:YT2824
- 应用:WB;IHC
- 种属:Human;Mouse
- 蛋白名称:
- M-phase phosphoprotein 9
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MPHOSPH9. AA range:539-588
- 特异性:
- MPP9 Polyclonal Antibody detects endogenous levels of MPP9 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MPHOSPH9;MPP9;M-phase phosphoprotein 9
- 背景:
- PTM:Phosphorylated in M (mitotic) phase.,
- 功能:
- PTM:Phosphorylated in M (mitotic) phase.,
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localizes to the distal and proximal end of centriole pairs in duplicated centrosomes. In ciliated cells, localizes to the distal and proximal end of daughter centriole and proximal of the mother centriole but not in the distal end of the mother centriole (PubMed:21399614). Recruited by KIF24 to the distal end of mother centriole where it forms a ring-like structure (PubMed:30375385). .
- 组织表达:
- Lymphoblast,Tongue,
- Western Blot analysis of various cells using MPP9 Polyclonal Antibody
- Western blot analysis of MPHOSPH9 Antibody. The lane on the right is blocked with the MPHOSPH9 peptide.
- Western blot analysis of the lysates from COLO205 cells using MPHOSPH9 antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).