MEL-1B-R Polyclonal Antibody
- 货号:YT2727
- 应用:WB;IHC
- 种属:Human
- 简介:
- >>Neuroactive ligand-receptor interaction;>>Circadian entrainment
- 蛋白名称:
- Melatonin receptor type 1B
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MTR1B. AA range:301-350
- 特异性:
- MEL-1B-R Polyclonal Antibody detects endogenous levels of MEL-1B-R protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MTNR1B;Melatonin receptor type 1B;Mel-1B-R;Mel1b receptor
- 背景:
- This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin. [provided by RefSeq, Jul 2008],
- 功能:
- function:High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.,online information:Melatonin receptor entry,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed in retina and less in brain and hippocampus.,
- 细胞定位:
- Cell membrane; Multi-pass membrane protein.
- 组织表达:
- Expressed in retina and less in brain and hippocampus.
![](/uploadfiles/PIC/yt2727-wb.jpg)
- Western Blot analysis of various cells using MEL-1B-R Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt2727-wb(2).jpg)
- Western Blot analysis of HepG2 cells using MEL-1B-R Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt2727_pic855018522.jpg)
- Western blot analysis of lysates from 293, Jurkat, and HepG2 cells, using MTR1B Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2727_pic1016118522.jpg)
- Western blot analysis of the lysates from HeLa cells using MTR1B antibody.
![](/uploadfiles/PIC/239ganaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).