MAP-4 Polyclonal Antibody
- 货号:YT2637
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Microtubule-associated protein 4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MAP4. AA range:662-711
- 特异性:
- MAP-4 Polyclonal Antibody detects endogenous levels of MAP-4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP4;Microtubule-associated protein 4;MAP-4
- 背景:
- The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
- 功能:
- alternative products:Additional isoforms seem to exist,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Non-neuronal microtubule-associated protein. Promotes microtubule assembly.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.,similarity:Contains 3 Tau/MAP repeats.,similarity:Contains 4 Tau/MAP repeats.,
- 细胞定位:
- Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center . Recruitment to microtubule is inhibited by microtubules polyglutamylation. .
- 组织表达:
- Bone marrow,Brain,Cerebellum,Colon carcinoma,Epithelium,Eye,Heart,Liver,Lym
![](/uploadfiles/PIC/yt2637_pic347218522.jpg)
- Immunofluorescence analysis of HeLa cells, using MAP4 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2637_pic347318522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAP4 Antibody. The picture on the right is blocked with the synthesized peptide.