LTB4DH Polyclonal Antibody
- 货号：YT2597
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Prostaglandin reductase 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PTGR1. AA range:75-124
- 特异性：
- LTB4DH Polyclonal Antibody detects endogenous levels of LTB4DH protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PTGR1;LTB4DH;Prostaglandin reductase 1;PRG-1;15-oxoprostaglandin 13-reductase;NADP-dependent leukotriene B4 12-hydroxydehydrogenase
- 背景：
- This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009],
- 功能：
- catalytic activity:11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)(+) = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.,catalytic activity:n-alkanal + NAD(P)(+) = alk-2-enal + NAD(P)H.,function:Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha (By similarity). Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.,similarity:Belongs to the NADP-dependent oxidoreductase L4BD family.,subunit:Monomer or homodimer.,tissue specificity:High expression in the kidney, liver, and intestine but not in leukocytes.,
- 组织表达：
- High expression in the kidney, liver, and intestine but not in leukocytes.
- Western Blot analysis of various cells using LTB4DH Polyclonal Antibody
- Western blot analysis of lysates from HT-29 cells, using PTGR1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from 293 cells using PTGR1 antibody.
