LPD lipase Polyclonal Antibody
- 货号：YT2586
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human LIPI. AA range:289-338
- 特异性：
- LPD lipase Polyclonal Antibody detects endogenous levels of LPD lipase protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- LIPI;LPDL;PRED5;Lipase member I;LIPI;Cancer/testis antigen 17;CT17;LPD lipase;Membrane-associated phosphatidic acid-selective phospholipase A1-beta;mPA-PLA1 beta
- 背景：
- The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
- 功能：
- disease:Defects in LIPI may be a cause of susceptibility to familial hypertrigliceridemia [MIM:145750]. Familial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.,enzyme regulation:Inhibited by sodium vanadate.,function:Hydrolyzes specifically phosphatidic acid (PA) to produce lysophosphatidic acid (LPA).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the AB hydrolase superfamily. Lipase family.,subcellular location:May associate with lipid draft.,subunit:Interacts with heparin with a high affinity.,tissue specificity:Expressed in testis. Expressed exclusively at the connecting piece of the sperm.,
- 细胞定位：
- [Isoform 1]: Cell membrane . Secreted . May associate with lipid draft. .; [Isoform 2]: Cell membrane . Secreted . May associate with lipid draft. .
- 组织表达：
- Expressed in testis. Expressed exclusively at the connecting piece of the sperm.
- Western Blot analysis of various cells using LPD lipase Polyclonal Antibody
- Western blot analysis of lysates from COLO cells, using LIPI Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using LIPI antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
