KKIAMRE Polyclonal Antibody
- 货号：YT2482
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Cyclin-dependent kinase-like 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CDKL2. AA range:211-260
- 特异性：
- KKIAMRE Polyclonal Antibody detects endogenous levels of KKIAMRE protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CDKL2;Cyclin-dependent kinase-like 2;Protein kinase p56 KKIAMRE;Serine/threonine-protein kinase KKIAMRE
- 背景：
- This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the cytoplasm, with lower levels in the nucleus. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The [NKR]KIAxRE motif seems to be a cyclin-binding region.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed in testis and kidney, and at lower level in brain and lung.,
- 细胞定位：
- Cytoplasm . Nucleus .
- 组织表达：
- Expressed in testis and kidney, and at lower level in brain and lung.
- Western blot analysis of lysates from Jurkat and 293 cells, using CDKL2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
