KKIALRE Polyclonal Antibody
- 货号:YT2481
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat
- 蛋白名称:
- Cyclin-dependent kinase-like 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CDKL1. AA range:281-330
- 特异性:
- KKIALRE Polyclonal Antibody detects endogenous levels of KKIALRE protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CDKL1;Cyclin-dependent kinase-like 1;Protein kinase p42 KKIALRE;Serine/threonine-protein kinase KKIALRE
- 背景:
- This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The [NKR]KIAxRE motif seems to be a cyclin-binding region.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highly expressed in kidney, and to a lower extent in ovary.,
- 细胞定位:
- Cytoplasm . Nucleus .
- 组织表达:
- Highly expressed in kidney, and to a lower extent in ovary.
- Western Blot analysis of various cells using KKIALRE Polyclonal Antibody diluted at 1:500
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from COLO205 cells, using CDKL1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using CDKL1 antibody.