KKIALRE Polyclonal Antibody

    • 货号:YT2481
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Rat
      • 靶点:
      • KKIALRE
      • 基因名称:
      • CDKL1
      • 蛋白名称:
      • Cyclin-dependent kinase-like 1
      • Human Gene Id:
      • 8814
      • Human Swiss Prot No:
      • Q00532
      • Mouse Swiss Prot No:
      • Q8CEQ0
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human CDKL1. AA range:281-330
      • 特异性:
      • KKIALRE Polyclonal Antibody detects endogenous levels of KKIALRE protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CDKL1;Cyclin-dependent kinase-like 1;Protein kinase p42 KKIALRE;Serine/threonine-protein kinase KKIALRE
      • 实测条带:
      • 42kD
      • 背景:
      • This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The [NKR]KIAxRE motif seems to be a cyclin-binding region.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highly expressed in kidney, and to a lower extent in ovary.,
      • 细胞定位:
      • Cytoplasm . Nucleus .
      • 组织表达:
      • Highly expressed in kidney, and to a lower extent in ovary.
      • 产品图片
      • Western Blot analysis of various cells using KKIALRE Polyclonal Antibody diluted at 1:500
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from COLO205 cells, using CDKL1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using CDKL1 antibody.