KIF20A Polyclonal Antibody
- 货号:YT2470
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Kinesin-like protein KIF20A
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human KIF20A. AA range:501-550
- 特异性:
- KIF20A Polyclonal Antibody detects endogenous levels of KIF20A protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- KIF20A;MKLP2;RAB6KIFL;Kinesin-like protein KIF20A;GG10_2;Mitotic kinesin-like protein 2;MKlp2;Rab6-interacting kinesin-like protein;Rabkinesin-6
- 背景:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- 功能:
- function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
- 细胞定位:
- Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Localizes to the spindle midzone during anaphase and telophase. .
- 组织表达:
- Brain,Endothelial cell,Epithelium,Uterus,
- Western Blot analysis of 293 cells using KIF20A Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunofluorescence analysis of HUVEC cells, using KIF20A Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KIF20A Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 cells, using KIF20A Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using KIF20A antibody.