• 靶点：
• IRS-1

• 简介：
• >>cGMP-PKG signaling pathway;>>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Neurotrophin signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Growth hormone synthesis, secretion and action;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>MicroRNAs in cancer;>>Diabetic cardiomyopathy

• 基因名称：
• IRS1

• 蛋白名称：
• Insulin receptor substrate 1

• Human Gene Id：
• 3667

• Human Swiss Prot No：
• P35568

• Mouse Gene Id：
• 16367

• Mouse Swiss Prot No：
• P35569

• Rat Gene Id：
• 25467

• Rat Swiss Prot No：
• P35570

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human IRS-1. AA range:603-652

• 特异性：
• IRS-1 Polyclonal Antibody detects endogenous levels of IRS-1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• IRS1;Insulin receptor substrate 1;IRS-1

• 实测条带：
• 170kD

• 背景：
• This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],

• 功能：
• disease:Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].,function:May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.,polymorphism:The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependen

• 细胞定位：
• nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle,

• 组织表达：
• Epithelium,Eye,Skeletal muscle,

Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC
货号：YT2404

Long Non-coding RNA GAS5 Maintains Insulin Secretion by Regulating Multiple miRNAs in INS-1 832/13 Cells. Frontiers in Molecular Biosciences Front Mol Biosci. 2020; 7: 559267 WB Rat 1 : 1000 INS-1 832/13 cell
货号：YT2404

Preventative effects of resveratrol and estradiol on streptozotocin-induced diabetes in ovariectomized mice and the related mechanisms. PLoS One 2018 Oct 01 WB Mouse Skeletal muscle,myocardial tissues
货号：YT2404

Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs. JOURNAL OF THERMAL BIOLOGY 2018 Aug 18 WB Pig 1:1000 Liver
货号：YT2404

Tao, Hong, et al. "MiR-126 suppresses the glucose-stimulated proliferation via IRS-2 in INS-1 β cells." PloS one 11.2 (2016): e0149954.
货号：YT2404

Oligosaccharides of Ophiopogon japonicus ameliorate insulin resistance and glucolipid metabolism in HFD/STZ-induced T2DM rats and IR-HepG2 cells via activation of the IRS-1/PI3K/AKT/GSK-3β pathway Journal of Functional Foods Baoting Chen WB Rat,Human liver tissue HepG2 cell
货号：YT2404

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