IP Receptor Polyclonal Antibody
- 货号：YT2378
- 应用：WB;ELISA;IHC
- 种属：Human;Rat;Mouse;
- 简介：
- >>Neuroactive ligand-receptor interaction;>>Vascular smooth muscle contraction;>>Platelet activation
- 蛋白名称：
- Prostacyclin receptor
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Prostacyclin Receptor. AA range:198-247
- 特异性：
- IP Receptor Polyclonal Antibody detects endogenous levels of IP Receptor protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PTGIR;PRIPR;Prostacyclin receptor;Prostaglandin I2 receptor;PGI receptor;PGI2 receptor;Prostanoid IP receptor
- 背景：
- The protein encoded by this gene is a member of the G-protein coupled receptor family 1 and has been shown to be a receptor for prostacyclin. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor. [provided by RefSeq, Jul 2008],
- 功能：
- function:Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.,PTM:Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.,PTM:Palmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.,similarity:Belongs to the G-protein coupled receptor 1 family.,
- 细胞定位：
- Cell membrane; Multi-pass membrane protein.
- 组织表达：
- Lung,Pancreas,Placenta,
- Immunofluorescence analysis of LOVO cells, using Prostacyclin Receptor Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from K562 cells, using Prostacyclin Receptor Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using Prostacyclin Receptor antibody.
- Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
