- 靶点：
- Id4
- 简介：
- >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
- 基因名称：
- ID4
- 蛋白名称：
- DNA-binding protein inhibitor ID-4
- Human Gene Id：
- 3400
- Human Swiss Prot No：
- P47928
- Mouse Gene Id：
- 15904
- Mouse Swiss Prot No：
- P41139
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ID4. AA range:1-50
- 特异性：
- Id4 Polyclonal Antibody detects endogenous levels of Id4 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ID4;BHLHB27;DNA-binding protein inhibitor ID-4;Class B basic helix-loop-helix protein 27;bHLHb27;Inhibitor of DNA binding 4
- 实测条带：
- 24kD
- 背景：
- This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011],
- 功能：
- function:ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Heterodimer with other HLH proteins.,
- 细胞定位：
- Nucleus.
- 组织表达：
- Abdominal adipose tissue,Uterus,
Polyamine metabolism links gut microbiota and testicular dysfunction. Microbiome Microbiome. 2021 Dec;9(1):1-18 IHC Mouse Testicular
货号：YT2272
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using ID4 antibody.
