- 靶点:
- HPK1
- 简介:
- >>MAPK signaling pathway
- 基因名称:
- MAP4K1
- 蛋白名称:
- Mitogen-activated protein kinase kinase kinase kinase 1
- Human Gene Id:
- 11184
- Human Swiss Prot No:
- Q92918
- Mouse Gene Id:
- 26411
- Mouse Swiss Prot No:
- P70218
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MEKKK 1. AA range:371-420
- 特异性:
- HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP4K1;HPK1;Mitogen-activated protein kinase kinase kinase kinase 1;Hematopoietic progenitor kinase;MAPK/ERK kinase kinase kinase 1;MEK kinase kinase 1;MEKKK 1
- 实测条带:
- 91kD
- 背景:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.,
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.,
- 细胞定位:
- intracellular,cytoplasm,membrane,
- 组织表达:
- Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1:1000
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 cells, using MEKKK 1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using MEKKK 1 antibody.