- 靶点:
- HNF4α
- 简介:
- >>AMPK signaling pathway;>>Maturity onset diabetes of the young
- 基因名称:
- HNF4A
- 蛋白名称:
- Hepatocyte nuclear factor 4-alpha
- Human Gene Id:
- 3172
- Human Swiss Prot No:
- P41235
- Mouse Gene Id:
- 15378
- Mouse Swiss Prot No:
- P49698
- Rat Gene Id:
- 25735
- Rat Swiss Prot No:
- P22449
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human HNF4 alpha. AA range:280-329
- 特异性:
- HNF-4α Polyclonal Antibody detects endogenous levels of HNF-4α protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HNF4A;HNF4;NR2A1;TCF14;Hepatocyte nuclear factor 4-alpha;HNF-4-alpha;Nuclear receptor subfamily 2 group A member 1;Transcription factor 14;TCF-14;Transcription factor HNF-4
- 实测条带:
- 52kD
- 背景:
- The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012],
- 功能:
- alternative products:Additional isoforms seem to exist,disease:Defects in HNF4A are the cause of maturity onset diabetes of the young type 1 (MODY1) [MIM:125850]; also shortened MODY-1. MODY [MIM:606391] is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age) and a primary defect in insulin secretion. The clinical phenotype of MODY1 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications.,function:Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.,miscellaneous:Binds fatty acids.,online information:Hepatocyte nuclear fac
- 细胞定位:
- Nucleus.
- 组织表达:
- Kidney,Liver,
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using HNF-4α Polyclonal Antibody diluted at 1:2000
- Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).