HisRS Polyclonal Antibody
- 货号：YT2139
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Aminoacyl-tRNA biosynthesis
- 蛋白名称：
- Histidine--tRNA ligase cytoplasmic
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509
- 特异性：
- HisRS Polyclonal Antibody detects endogenous levels of HisRS protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- HARS;HRS;Histidine--tRNA ligase; cytoplasmic;Histidyl-tRNA synthetase;HisRS
- 背景：
- Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],
- 功能：
- catalytic activity:ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:Contains 1 WHEP-TRS domain.,tissue specificity:Brain, heart, liver and kidney.,
- 组织表达：
- Brain, heart, liver and kidney.
- Western Blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1:2000
- Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
