HDAC8 Polyclonal Antibody
- 货号:YT2120
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat;Monkey
- 简介:
- >>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis
- 蛋白名称:
- Histone deacetylase 8
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human HDAC8. AA range:5-54
- 特异性:
- HDAC8 Polyclonal Antibody detects endogenous levels of HDAC8 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HDAC8;HDACL1;CDA07;Histone deacetylase 8;HD8
- 背景:
- Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009],
- 功能:
- catalytic activity:Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.,miscellaneous:Its activity is inhibited by trichostatin A (TSA) and butyrate, two well known histone deacetylase inhibitors.,similarity:Belongs to the histone deacetylase family. Type 1 subfamily.,subcellular location:Excluded from the nucleoli.,subunit:Interacts with PEPB2-MYH11, a f
- 细胞定位:
- Nucleus . Chromosome . Cytoplasm . Excluded from the nucleoli (PubMed:10748112). Found in the cytoplasm of cells showing smooth muscle differentiation (PubMed:15772115, PubMed:16538051). .
- 组织表达:
- Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
![](/uploadfiles/PIC/yt2120-wb.jpg)
- Western Blot analysis of various cells using HDAC8 Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt2120-wb(2).jpg)
- Western Blot analysis of 293-UV cells using HDAC8 Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt2120_pic378218522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HDAC8 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2120_pic378318522.jpg)
- Western blot analysis of lysates from NIH/3T3, HeLa, and Jurkat cells, , using HDAC8 Antibody. The lane on the right is blocked with the synthesized peptide.