GRK 5 Polyclonal Antibody
- 货号：YT2070
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Chemokine signaling pathway;>>Endocytosis;>>Morphine addiction
- 蛋白名称：
- G protein-coupled receptor kinase 5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GRK5. AA range:351-400
- 特异性：
- GRK 5 Polyclonal Antibody detects endogenous levels of GRK 5 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GRK5;GPRK5;G protein-coupled receptor kinase 5;G protein-coupled receptor kinase GRK5
- 背景：
- This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.,function:Specifically phosphorylates the activated forms of G protein-coupled receptors.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,tissue specificity:Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.,
- 细胞定位：
- Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
- 组织表达：
- Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
- Western Blot analysis of various cells using GRK 5 Polyclonal Antibody
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- Western Blot analysis of COLO205 cells using GRK 5 Polyclonal Antibody
- Western blot analysis of lysates from HeLa, HepG2, and HUVEC cells, using GRK5 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
