GRB14 Polyclonal Antibody
- 货号:YT2055
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Growth factor receptor-bound protein 14
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GRB14. AA range:81-130
- 特异性:
- GRB14 Polyclonal Antibody detects endogenous levels of GRB14 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GRB14;Growth factor receptor-bound protein 14;GRB14 adapter protein
- 背景:
- The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
- 功能:
- function:Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain.,PTM:Phosphorylated on serine residues.,similarity:Belongs to the GRB7/10/14 family.,similarity:Contains 1 PH domain.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,subunit:Binds to the ankyrin repeat region of TNKS2 via its N-terminus.,tissue specificity:Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.,
- 细胞定位:
- Cytoplasm . Endosome membrane ; Peripheral membrane protein . Upon insulin stimulation, translocates to the plasma membrane. .
- 组织表达:
- Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
![](/uploadfiles/PIC/yt2055-wb.jpg)
- Western Blot analysis of various cells using GRB14 Polyclonal Antibody
![](/uploadfiles/PIC/yt2055_pic700618522.jpg)
- Western blot analysis of lysates from HUVEC, COLO, and MCF-7 cells, using GRB14 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/23-ihc-xiaochangaic20220712.jpg)
- Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200