GPR27 Polyclonal Antibody
- 货号：YT2011
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Probable G-protein coupled receptor 27
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GPR27. AA range:181-230
- 特异性：
- GPR27 Polyclonal Antibody detects endogenous levels of GPR27 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GPR27;SREB1;Probable G-protein coupled receptor 27;Super conserved receptor expressed in brain 1
- 背景：
- GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010],
- 功能：
- function:Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein .
- 组织表达：
- Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum.
- Immunofluorescence analysis of A549 cells, using GPR27 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using GPR27 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GPR27 antibody.
