- 靶点：
- GPR116
- 基因名称：
- GPR116
- 蛋白名称：
- Probable G-protein coupled receptor 116
- Human Gene Id：
- 221395
- Human Swiss Prot No：
- Q8IZF2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GPR116. AA range:11-60
- 特异性：
- GPR116 Polyclonal Antibody detects endogenous levels of GPR116 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GPR116;KIAA0758;Probable G-protein coupled receptor 116
- 实测条带：
- 150kD
- 背景：
- function:May have a role in the regulation of acid-base balance.,PTM:Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.,similarity:Contains 1 GPS domain.,similarity:Contains 1 SEA domain.,similarity:Contains 3 Ig-like (immunoglobulin-like) domains.,subunit:Exists as disulfide-linked dimers at the cell surface.,
- 功能：
- function:May have a role in the regulation of acid-base balance.,PTM:Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.,similarity:Contains 1 GPS domain.,similarity:Contains 1 SEA domain.,similarity:Contains 3 Ig-like (immunoglobulin-like) domains.,subunit:Exists as disulfide-linked dimers at the cell surface.,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein .
- 组织表达：
- Brain,Plasma,Testis,
Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers. Weimin Li IHC Human lung adenocarcinoma (LUAD) tissue,lung tissue
货号：YT1960
- June 19-2018
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- Antibody-FAQs
- 产品图片
- Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers. Weimin Li IHC Human lung adenocarcinoma (LUAD) tissue,lung tissue
- Western Blot analysis of various cells using GPR116 Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of HeLa cells, using GPR116 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from A549 cells, using GPR116 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GPR116 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
