Glucosidase IIβ Polyclonal Antibody
- 货号：YT1919
- 应用：WB;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Protein processing in endoplasmic reticulum
- 蛋白名称：
- Glucosidase 2 subunit beta
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GLU2B. AA range:81-130
- 特异性：
- Glucosidase IIβ Polyclonal Antibody detects endogenous levels of Glucosidase IIβ protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PRKCSH;G19P1;Glucosidase 2 subunit beta;80K-H protein;Glucosidase II subunit beta;Protein kinase C substrate 60.1 kDa protein heavy chain;PKCSH
- 背景：
- This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
- 功能：
- disease:Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.,function:Regulatory subunit of glucosidase II.,pathway:Glycan metabolism; N-glycan metabolism.,similarity:Contains 1 PRKCSH domain.,similarity:Contains 2 EF-hand domains.,subunit:Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.,
- 细胞定位：
- Endoplasmic reticulum .
- 组织表达：
- Lung,Lymphocyte,Platelet,
- Western Blot analysis of various cells using Glucosidase IIβ Polyclonal Antibody
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- Western Blot analysis of A549 cells using Glucosidase IIβ Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using GLU2B Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2, 293, and HUVEC cells, using GLU2B Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from 293 cells using GLU2B antibody.
