- 靶点：
- Frizzled-7
- 简介：
- >>mTOR signaling pathway;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
- 基因名称：
- FZD7
- 蛋白名称：
- Frizzled-7
- Human Gene Id：
- 8324
- Human Swiss Prot No：
- O75084
- Mouse Gene Id：
- 14369
- Mouse Swiss Prot No：
- Q61090
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94
- 特异性：
- Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;Flow Cyt 1:50-200;IF ICC 1:100-500;ELISA 1:5000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- FZD7;Frizzled-7;Fz-7;hFz7;FzE3
- 实测条带：
- 64kD
- 背景：
- frizzled class receptor 7(FZD7) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008],
- 功能：
- domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions. .
- 组织表达：
- High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
Effects of polyethylene oxide and poly(3-hydroxybutyrate- co -3-hydroxyvalerate) nanofibrous substrate on omental adipose-derived mesenchymal stem cell neuronal differentiation and peripheral nerve regeneration. RSC Advances Rsc Adv. 2017 Sep;7(68):42833-42844 WB Rat omental adipose-derived mesenchymal stem cells (O-ASCs)
货号：YT1786
The expression and function of Frizzled-7 in human renal cell carcinoma. Clinical & Translational Oncology Clin Transl Oncol. 2016 Mar;18(3):269-276 FC Human 786-O cell, OS-RC-2 cell, Caki-1 cell
货号：YT1786
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- Antibody-FAQs
- 产品图片
- Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
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- Western Blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
- Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HT-29 cells, using FZD7 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using FZD7 antibody.
