Flotillin-2 Polyclonal Antibody
- 货号:YT1727
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Insulin signaling pathway
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human Flotillin-2. AA range:95-144
- 特异性:
- Flotillin-2 Polyclonal Antibody detects endogenous levels of Flotillin-2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FLOT2;ESA1;M17S1;Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1
- 背景:
- Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008],
- 功能:
- function:May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.,similarity:Belongs to the band 7/mec-2 family. Flotillin subfamily.,subcellular location:Membrane-associated protein of caveolae.,subunit:Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2.,tissue specificity:In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.,
- 细胞定位:
- Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane ; Lipid-anchor . Membrane-associated protein of caveolae.
- 组织表达:
- In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
![](/uploadfiles/PIC/yt1727-wb.jpg)
- Western Blot analysis of various cells using Flotillin-2 Polyclonal Antibody
![](/uploadfiles/PIC/yt1727_pic928418522.jpg)
- Western blot analysis of lysate from A549 cells, using Flotillin-2 antibody.
![](/uploadfiles/PIC/ym3484-flotillin-2-001.jpg)
- Western blot analysis of 1) A431, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with Flotllin-2 Rabbit pAb diluted at 1:2,000.
![](/uploadfiles/PIC/ym3484-flotillin-2-002.jpg)
- Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using Flotillin-2 Rabbit pAb diluted at 1:200