ERGIC-3 Polyclonal Antibody
- 货号:YT1620
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Endoplasmic reticulum-Golgi intermediate compartment protein 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ERGI3. AA range:321-370
- 特异性:
- ERGIC-3 Polyclonal Antibody detects endogenous levels of ERGIC-3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ERGIC3;C20orf47;ERV46;SDBCAG84;CGI-54;Endoplasmic reticulum-Golgi intermediate compartment protein 3;Serologically defined breast cancer antigen NY-BR-84
- 背景:
- function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
- 功能:
- function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32.,
- 细胞定位:
- Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cycles between the endoplasmic reticulum and the Golgi.
- 组织表达:
- Brain,Embryo,Fetal liver,Liver,Mammary gland,Skin,
- Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from LOVO and NIH/3T3 cells, using ERGI3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).