- 靶点：
- ErbB-3/her2
- 简介：
- >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>Proteoglycans in cancer;>>MicroRNAs in cancer
- 基因名称：
- ERBB3,HER3
- 蛋白名称：
- Receptor tyrosine-protein kinase erbB-3
- Human Gene Id：
- 2065
- Human Swiss Prot No：
- P21860
- Mouse Gene Id：
- 13867
- Mouse Swiss Prot No：
- Q61526
- Rat Gene Id：
- 29496
- Rat Swiss Prot No：
- Q62799
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human HER3. AA range:1293-1342
- 特异性：
- ErbB-3 Polyclonal Antibody detects endogenous levels of ErbB-3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ERBB3;HER3;Receptor tyrosine-protein kinase erbB-3;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3
- 实测条带：
- 150-210kD
- 背景：
- This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the m
- 功能：
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.,disease:Overexpressed in a subset of human mammary tumors.,domain:The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.,function:Binds and is activated by neuregulins and NTAK.,PTM:Li
- 细胞定位：
- [Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
- 组织表达：
- Epithelial tissues and brain.
HSP90 inhibitor AUY922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells. CANCER SCIENCE 2017 May 19 WB Human MCF-7 cell，T47D cell
货号：YT1608
A novel multi-target inhibitor harboring selectivity of inhibiting EGFR T790M sparing wild-type EGFR. American Journal of Cancer Research Am J Cancer Res. 2017; 7(9): 1884–1898 WB Human NCI-H1975 cell,A549 cell
货号：YT1608
GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex Biomolecules Baikang Xie WB Human 1:1000 platelets,erythrocytes,leukocytes
货号：YT1608
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- Antibody-FAQs
- 产品图片
- Chen, Tingting, et al. "Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma." Oncology letters 14.5 (2017): 5934-5946.
- Western Blot analysis of various cells using ErbB-3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from HeLa cells, using HER3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using HER3 antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
