Endo G-L1 Polyclonal Antibody
- 货号：YT1555
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Nuclease EXOG mitochondrial
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ENDOGL1. AA range:171-220
- 特异性：
- Endo G-L1 Polyclonal Antibody detects endogenous levels of Endo G-L1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EXOG;ENDOGL1;ENDOGL2;ENGL;Nuclease EXOG; mitochondrial;Endonuclease G-like 1;Endo G-like 1
- 背景：
- This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18. [provided by RefSeq, Feb 2009],
- 功能：
- cofactor:Divalent metal cations.,function:Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single stranded DNA and 5'-3' exonuclease activity.,miscellaneous:The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.,similarity:Belongs to the DNA/RNA non-specific endonuclease family.,subunit:Homodimer.,tissue specificity:Ubiquitous.,
- 细胞定位：
- Mitochondrion inner membrane .
- Western Blot analysis of various cells using Endo G-L1 Polyclonal Antibody
- Western blot analysis of lysates from HUVEC, HT-29, and HepG2 cells, using ENDOGL1 Antibody. The lane on the right is blocked with the synthesized peptide.
