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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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EMR4 Polyclonal Antibody
- 货号:YT1548
- 应用:WB;IF;ELISA
- 种属:Human;Monkey
- 蛋白名称:
- Putative EGF-like module-containing mucin-like hormone receptor-like 4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human EMR4P. AA range:101-150
- 特异性:
- EMR4 Polyclonal Antibody detects endogenous levels of EMR4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EMR4P;EMR4;GPR127;PGR16;Putative EGF-like module-containing mucin-like hormone receptor-like 4;EGF-like module receptor 4;G-protein coupled receptor 127;G-protein coupled receptor PGR16
- 背景:
- This gene is a member of the EGF-TM7 receptor gene family which is thought to play a role in leukocyte adhesion and migration. In other vertebrates, including nonhuman primates, this gene encodes a protein containing N-terminal EGF domains and a C-terminal transmembrane domain. Sequence evidence for the human gene, however, indicates nucleotide deletion in the genomic sequence would result in frameshift and early termination of translation. A protein expressed by this gene would be soluble rather than expressed on the cell surface. As the encoded protein has not been detected, this gene may represent a transcribed pseudogene. [provided by RefSeq, Aug 2008],
- 细胞定位:
- [Isoform 1]: Cell membrane ; Multi-pass membrane protein .; [Isoform 2]: Secreted .
- Western Blot analysis of various cells using EMR4 Polyclonal Antibody
- Western Blot analysis of COLO205 cells using EMR4 Polyclonal Antibody
- Western blot analysis of lysates from HUVEC cells, COS7 cells, HeLa cells, and COLO205 cells, using EMR4 Antibody. The lane on the right is blocked with the synthesized peptide.