Egr-2 Polyclonal Antibody
- 货号:YT1498
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Apelin signaling pathway;>>GnRH signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>AGE-RAGE signaling pathway in diabetic complications;>>Prion disease;>>Human T-cell leukemia virus 1 infection
- 蛋白名称:
- Early growth response protein 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human EGR1/2. AA range:371-420
- 特异性:
- Egr-2 Polyclonal Antibody detects endogenous levels of Egr-2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EGR1;KROX24;ZNF225;Early growth response protein 1;EGR-1;AT225;Nerve growth factor-induced protein A;NGFI-A;Transcription factor ETR103;Transcription factor Zif268;Zinc finger protein 225;Zinc finger protein Krox-24;EGR2;KROX20
- 背景:
- The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014],
- 功能:
- function:Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.,induction:By growth factors.,similarity:Belongs to the EGR C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,
- 细胞定位:
- Nucleus . Cytoplasm .
- 组织表达:
- Detected in neutrophils (at protein level).
- Western Blot analysis of various cells using Egr-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from HeLa and Jurkat cells, using EGR1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).