DUS2L Polyclonal Antibody
- 货号:YT1422
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DUS2L. AA range:421-470
- 特异性:
- DUS2L Polyclonal Antibody detects endogenous levels of DUS2L protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DUS2L;DUS2;tRNA-dihydrouridine(20) synthase [NAD(P)+]-like;Up-regulated in lung cancer protein 8;URLC8;tRNA-dihydrouridine synthase 2-like;hDUS2
- 背景:
- dihydrouridine synthase 2(DUS2) Homo sapiens This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012],
- 功能:
- cofactor:FAD.,function:Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.,similarity:Belongs to the dus family. Dus2 subfamily.,similarity:Contains 1 DRBM (double-stranded RNA-binding) domain.,subcellular location:Mainly at the endoplasmic reticulum.,subunit:Interacts with EPRS.,tissue specificity:Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).,
- 细胞定位:
- Cytoplasm . Endoplasmic reticulum . Mainly at the endoplasmic reticulum. .
- 组织表达:
- Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).
- Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using DUS2L Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from RAW264.7 cells, using DUS2L Antibody. The lane on the right is blocked with the synthesized peptide.