CLC-6 Polyclonal Antibody
- 货号：YT0960
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat;Monkey
- 蛋白名称：
- Chloride transport protein 6
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CLCN6. AA range:611-660
- 特异性：
- CLC-6 Polyclonal Antibody detects endogenous levels of CLC-6 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CLCN6;KIAA0046;Chloride transport protein 6;Chloride channel protein 6;ClC-6
- 背景：
- chloride voltage-gated channel 6(CLCN6) Homo sapiens This gene encodes a member of the voltage-dependent chloride channel protein family. Members of this family can function as either chloride channels or antiporters. This protein is primarily localized to late endosomes and functions as a chloride/proton antiporter. Alternate splicing results in both coding and non-coding variants. Additional alternately spliced variants have been described but their full-length structure is unknown. [provided by RefSeq, Mar 2012],
- 功能：
- function:Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter.,miscellaneous:The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.,PTM:N-glycosylated on several asparagine residues.,similarity:Belongs to the chloride channel (TC 2.A.49) family.,similarity:Contains 2 CBS domains.,subcellular location:Detected in detergent-resistant lipid rafts.,tissue specificity:Testis, ovary, small intestine, brain and skeletal muscle. Low level expression in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Isoform C is only detected in kidney.,
- 细胞定位：
- Late endosome membrane ; Multi-pass membrane protein .
- 组织表达：
- Testis, ovary, small intestine, brain and skeletal muscle. Low level expression in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Isoform 3 is only detected in kidney.
- Western Blot analysis of various cells using CLC-6 Polyclonal Antibody diluted at 1:500
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- Western Blot analysis of L929 cells using CLC-6 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from COS7 and HT-29 cells, using CLCN6 Antibody. The lane on the right is blocked with the synthesized peptide.
