Centrobin Polyclonal Antibody
- 货号：YT0855
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CNTROB. AA range:591-640
- 特异性：
- Centrobin Polyclonal Antibody detects endogenous levels of Centrobin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CNTROB;LIP8;PP1221;Centrobin;Centrosomal BRCA2-interacting protein;LYST-interacting protein 8
- 背景：
- This gene encodes a centrosomal protein that interacts with BRCA2, and is required for centriole duplication and cytokinesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011],
- 功能：
- developmental stage:Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases.,function:Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Wrong choice of frame.,subcellular location:Centriole-associated, asymmetrically localizes to the daughter centriole.,subunit:Interacts with LYST.,tissue specificity:Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.,
- 细胞定位：
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Centriole-associated, asymmetrically localizes to the daughter centriole.
- 组织表达：
- Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
- Western Blot analysis of various cells using Centrobin Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain, using CNTROB Antibody. The picture on the right is blocked with the synthesized peptide.
