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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CCRK Polyclonal Antibody
- 货号:YT0711
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Cyclin-dependent kinase 20
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CCRK. AA range:31-80
- 特异性:
- CCRK Polyclonal Antibody detects endogenous levels of CCRK protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CDK20;CCRK;CDCH;Cyclin-dependent kinase 20;CDK-activating kinase p42;CAK-kinase p42;Cell cycle-related kinase;Cell division protein kinase 20;Cyclin-dependent protein kinase H;Cyclin-kinase-activating kinase p42
- 背景:
- The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase may activate cyclin-dependent kinase 2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Dec 2009],
- 功能:
- similarity:Belongs to the protein kinase superfamily.,
- 细胞定位:
- Nucleus . Cytoplasm . Cell projection, cilium .
- Immunofluorescence analysis of HUVEC cells, using CCRK Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from RAW264.7 cells, using CCRK Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from RAW264.7cells using CCRK antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).