Casein Kinase Iγ2 Polyclonal Antibody

    • 货号:YT0649
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Casein Kinase Iγ2
      • 简介:
      • >>Hedgehog signaling pathway
      • 基因名称:
      • CSNK1G2
      • 蛋白名称:
      • Casein kinase I isoform gamma-2
      • Human Gene Id:
      • 1455
      • Human Swiss Prot No:
      • P78368
      • Mouse Swiss Prot No:
      • Q8BVP5
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human CKI-gamma2. AA range:10-59
      • 特异性:
      • Casein Kinase Iγ2 Polyclonal Antibody detects endogenous levels of Casein Kinase Iγ2 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CSNK1G2;CK1G2;Casein kinase I isoform gamma-2;CKI-gamma 2
      • 实测条带:
      • 47kD
      • 背景:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer.,tissue specificity:Testis.,
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer.,tissue specificity:Testis.,
      • 细胞定位:
      • Cytoplasm, cell cortex . Cytoplasm .
      • 组织表达:
      • Testis.

      The Catalytically Inactive Mutation of the Ubiquitin-Conjugating Enzyme CDC34 Affects its Stability and Cell Proliferation. PROTEIN JOURNAL Protein J. 2018 Apr;37(2):132-143 WB Human HEK293T cell, HeLa cell
      货号:YT0649

      • 产品图片
      • Western Blot analysis of HeLa cells using Casein Kinase Iγ2 Polyclonal Antibody
      • Western blot analysis of lysates from HeLa and 293 cells, using CKI-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).