- 靶点:
- CaMK2
- 简介:
- >>ErbB signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>HIF-1 signaling pathway;>>Oocyte meiosis;>>Necroptosis;>>Adrenergic signaling in cardiomyocytes;>>Wnt signaling pathway;>>Axon guidance;>>Circadian entrainment;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Dopaminergic synapse;>>Olfactory transduction;>>Inflammatory mediator regulation of TRP channels;>>Insulin secretion;>>GnRH signaling pathway;>>Melanogenesis;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Aldosterone synthesis and secretion;>>Cushing syndrome;>>Gastric acid secretion;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases;>>Amphetamine addiction;>>Tuberculosis;>>Pathways in cancer;>>Proteoglycans in cancer;>>Glioma;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis
- 基因名称:
- CAMK2A/CAMK2D
- 蛋白名称:
- Calcium/calmodulin-dependent protein kinase type II subunit alpha/delta
- Human Gene Id:
- 815/817
- Human Swiss Prot No:
- Q9UQM7/Q13557
- Mouse Gene Id:
- 12322/108058
- Rat Gene Id:
- 25400/24246
- Rat Swiss Prot No:
- P11275/P15791
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CaMK2 alpha/delta. AA range:256-305
- 特异性:
- CaMKIIα/δ Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CAMK2A;CAMKA;KIAA0968;Calcium/calmodulin-dependent protein kinase type II subunit alpha;CaM kinase II subunit alpha;CaMK-II subunit alpha;CAMK2D;CAMKD;Calcium/calmodulin-dependent protein kinase type II subunit delta;CaM kinase II
- 实测条带:
- 54kD
- 背景:
- The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.,function:CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Postsynaptic lipid rafts.,subunit:CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric
- 细胞定位:
- Cell junction, synapse . Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, dendrite . Postsynaptic lipid rafts. .
- 组织表达:
- Brain,
NETO2 promotes melanoma progression via activation of the Ca2+/CaMKII signaling pathway Frontiers of Medicine Cong Peng WB Human SK-Mel-5 cell,SK-Mel-28 cell, WM35 cell
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- 产品图片
- NETO2 promotes melanoma progression via activation of the Ca2+/CaMKII signaling pathway Frontiers of Medicine Cong Peng WB Human SK-Mel-5 cell,SK-Mel-28 cell, WM35 cell
- Western Blot analysis of NIH-3T3/K562 cells using CaMKIIα/δ Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of MCF7 cells, using CaMK2 alpha/delta Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CaMK2 alpha/delta Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 and K562 cells, using CaMK2 alpha/delta Antibody. The lane on the right is blocked with the synthesized peptide.