• 靶点：
• Calpain 2

• 简介：
• >>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Necroptosis;>>Cellular senescence;>>Focal adhesion;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis

• 基因名称：
• CAPN2

• 蛋白名称：
• Calpain-2 catalytic subunit

• Human Gene Id：
• 824

• Human Swiss Prot No：
• P17655

• Mouse Gene Id：
• 12334

• Mouse Swiss Prot No：
• O08529

• Rat Gene Id：
• 29154

• Rat Swiss Prot No：
• Q07009

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Calpain 2. AA range:305-354

• 特异性：
• Calpain 2 Polyclonal Antibody detects endogenous levels of Calpain 2 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CAPN2;CANPL2;Calpain-2 catalytic subunit;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain

• 分子量：
• 80kD

• 背景：
• The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

• 功能：
• catalytic activity:Broad endopeptidase specificity.,cofactor:Binds 3 calcium ions.,enzyme regulation:Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.,function:Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the peptidase C2 family.,similarity:Contains 1 calpain catalytic domain.,similarity:Contains 3 EF-hand domains.,subcellular location:Translocates to the plasma membrane upon Ca(2+) binding.,subunit:Forms a heterodimer with a small (regulatory) subunit (CAPNS1).,tissue specificity:Ubiquitous.,

• 细胞定位：
• Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.

• 组织表达：
• Ubiquitous.

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