Cadherin-9 Polyclonal Antibody
- 货号:YT0604
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CDH9. AA range:201-250
- 特异性:
- Cadherin-9 Polyclonal Antibody detects endogenous levels of Cadherin-9 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq, Jul 2008],
- 功能:
- function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.,similarity:Contains 5 cadherin domains.,
- 细胞定位:
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达:
- PCR rescued clones,
- Western Blot analysis of various cells using Cadherin-9 Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using CDH9 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDH9 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COLO cells, using CDH9 Antibody. The lane on the right is blocked with the synthesized peptide.