BRAF35 Polyclonal Antibody
- 货号：YT0515
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;
- 蛋白名称：
- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human HMG20B. AA range:1-50
- 特异性：
- BRAF35 Polyclonal Antibody detects endogenous levels of BRAF35 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- HMG20B;BRAF35;HMGX2;HMGXB2;SMARCE1R;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related;SMARCE1-related protein;BRCA2-associated factor 35;HMG box-containing protein 20B;HMG domain-
- 背景：
- function:Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Localized to condensed chromosomes in mitosis in conjunction with BRCA2.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein.,tissue specificity:Ubiquitously expressed in adult tissues.,
- 功能：
- function:Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Localized to condensed chromosomes in mitosis in conjunction with BRCA2.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein.,tissue specificity:Ubiquitously expressed in adult tissues.,
- 细胞定位：
- Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
- 组织表达：
- Ubiquitously expressed in adult tissues.
- Western Blot analysis of various cells using BRAF35 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of AD293 cells using BRAF35 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat cells, using HMG20B Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
