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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ApoF Polyclonal Antibody
- 货号:YT0276
- 应用:WB;ELISA
- 种属:Human;Rat;Mouse;
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human APOF. AA range:221-270
- 特异性:
- ApoF Polyclonal Antibody detects endogenous levels of ApoF protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- APOF;Apolipoprotein F;Apo-F;Lipid transfer inhibitor protein;LTIP
- 背景:
- The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008],
- 功能:
- function:Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.,tissue specificity:Liver. Secreted in plasma.,
- 组织表达:
- Expressed by the liver and secreted in plasma.
- Western Blot analysis of various cells using ApoF Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of A549 cells using ApoF Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from HeLa, Jurkat, COLO, and A549 cells, using APOF Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using APOF antibody.