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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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AIFL Polyclonal Antibody
- 货号:YT0148
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Apoptosis-inducing factor 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human AIFM3. AA range:10-59
- 特异性:
- AIFL Polyclonal Antibody detects endogenous levels of AIFL protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- AIFM3;AIFL;Apoptosis-inducing factor 3;Apoptosis-inducing factor-like protein
- 背景:
- domain:The Rieske domain induces apoptosis.,function:Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 Rieske domain.,subcellular location:Does not translocate to the nucleus upon induction of apoptosis.,tissue specificity:Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).,
- 功能:
- domain:The Rieske domain induces apoptosis.,function:Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 Rieske domain.,subcellular location:Does not translocate to the nucleus upon induction of apoptosis.,tissue specificity:Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).,
- 细胞定位:
- Mitochondrion . Does not translocate to the nucleus upon induction of apoptosis.
- 组织表达:
- Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).
![](/uploadfiles/PIC/yt0148-wb.jpg)
- Western Blot analysis of various cells using AIFL Polyclonal Antibody
![](/uploadfiles/PIC/yt0148_pic598918522.jpg)
- Immunofluorescence analysis of A549 cells, using AIFM3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0148_pic599018522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain, using AIFM3 Antibody. The picture on the right is blocked with the synthesized peptide.