Actinin-α3 Polyclonal Antibody
- 货号：YT0103
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Arrhythmogenic right ventricular cardiomyopathy
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ACTN3. AA range:1-50
- 特异性：
- Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ACTN3;Alpha-actinin-3;Alpha-actinin skeletal muscle isoform 3;F-actin cross-linking protein
- 背景：
- This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014],
- 功能：
- function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,polymorphism:About 18% of the world population lack a functional ACTN3 due to a stop codon polymorphism at position 577. The absence of a functional ACTN3 expression is not correlated with a disease state.,sequence caution:According to the human genome assembly there is a stop codon in position 577 which is only found in 18% of the world population.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.,tissue specificity:Expressed only in a subset of type 2 skeletal muscle fibers.,
- 细胞定位：
- intracellular,cytosol,actin filament,brush border,focal adhesion,Z disc,pseudopodium,extracellular exosome,
- 组织表达：
- Expressed only in a subset of type 2 skeletal muscle fibers.
- Western Blot analysis of various cells using Actinin-α3 Polyclonal Antibody
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- Western Blot analysis of HT29 cells using Actinin-α3 Polyclonal Antibody
- Western blot analysis of lysates from HT-29 cells, using ACTN3 Antibody. The lane on the right is blocked with the synthesized peptide.
