• 靶点：
• 3β-HSD7

• 简介：
• >>Primary bile acid biosynthesis;>>Metabolic pathways

• 基因名称：
• HSD3B7

• 蛋白名称：
• 3 beta-hydroxysteroid dehydrogenase type 7

• Human Gene Id：
• 80270

• Human Swiss Prot No：
• Q9H2F3

• Mouse Gene Id：
• 101502

• Mouse Swiss Prot No：
• Q9EQC1

• Rat Gene Id：
• 246211

• Rat Swiss Prot No：
• O35048

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human HSD3B7. AA range:121-170

• 特异性：
• 3β-HSD7 Polyclonal Antibody detects endogenous levels of 3β-HSD7 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HSD3B7;3 beta-hydroxysteroid dehydrogenase type 7;3 beta-hydroxysteroid dehydrogenase type VII;3-beta-HSD VII;3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase;C(27) 3-beta-HSD;Cholest-5-ene-3-beta;7-alpha-diol 3-beta-dehydrogenase

• 实测条带：
• 41kD

• 背景：
• This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

• 功能：
• catalytic activity:3-beta-hydroxy-Delta(5)-steroid + NAD(+) = 3-oxo-Delta(5)-steroid + NADH.,catalytic activity:Cholest-5-ene-3-beta,7-alpha-diol + NAD(+) = 7-alpha-hydroxycholest-4-en-3-one + NADH.,disease:Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1) [MIM:607765]; also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis.,function:Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests tha

• 细胞定位：
• Endoplasmic reticulum membrane; Multi-pass membrane protein.

• 组织表达：
• Stomach,Testis,Uterus,

Determination of cadmium induced acute and chronic reproductive toxicity with Raman spectroscopy. LASERS IN MEDICAL SCIENCE Laser Med Sci. 2020 Dec;35(9):1919-1926 WB Mouse testis
货号：YT0015

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