• 靶点：
• SF3B1

• 简介：
• >>Spliceosome

• 基因名称：
• SF3B1 SAP155

• 蛋白名称：
• SF3B1 (Thr313)

• Human Gene Id：
• 23451

• Human Swiss Prot No：
• O75533

• Mouse Swiss Prot No：
• Q99NB9

• 免疫原：
• Synthesized phosho peptide around human SF3B1 (Thr313)

• 特异性：
• This antibody detects endogenous levels of Human Mouse SF3B1 (phospho-Thr313)

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000;IHC 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Splicing factor 3B subunit 1 (Pre-mRNA-splicing factor SF3b 155 kDa subunit) (SF3b155) (Spliceosome-associated protein 155) (SAP 155)

• 实测条带：
• 145kD

• 背景：
• This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

• 功能：
• function:Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.,PTM:Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.,similarity:Belongs to the SF3B1 family.,similarity:Contains 11 HEAT repeats.,subcellular location:During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.,subunit:Identified

• 细胞定位：
• Nucleus . Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

• 组织表达：
• Brain,Epithelium,Pooled,Testis,Uterus,

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